in vitro試験

In vitro testing

Safety testing (an alternative to animal testing)

Eye irritation testing through the use of SIRC-STE method, OECD TG 491 method and ingredients

The Short Time Exposure in vitroTesting is listed on the OECD testing guidelines (TG491) as the alternative method for the Draize Rabbit Eye Irritation Test.
It tests for eye irritancy according to the cytotoxicity index of chemicals on SIRC cells.

Eye irritation testing using the SIRC-NRU method

We evaluate the potential eye corrosivity/irritation of the substances in the product on a sample rabbit cornea epithelial cell by conducting a cytotoxicity test (NR assay).

BCOP (OECD TG437 method; ingredients)

We evaluate the potential eye corrosivity/irritation of the substances in the product by measuring the permeability variation quantitatively after application on a bovine corneal enucleated from the eyeball
This test is listed on the OECD test guidelines (TG437) as an alternative method for testing the potential eye corrosivity/irritation of substances in a product (Draize test) using rabbit eye as a base material.

BCOP (Original method; formulation)

We test the potential eye corrosivity/irritation of substances in a product by modifying BCOP (OECD TG437 test) into an evaluable method of the formulation.
It is possible to compare the potential eye corrosivity/irritation of substances in a product’s formulation similar to that of the testing done in the EU market.

Eye mucous membrane irritation tests of three-dimensional models (OECD TG492 method; mterials)

We measure the potential eye corrosivity/irritation of substances in a product by using a three-dimensional cultured epidermis model (MatTek corporation EpiDerm™), which is restructured by a normal keratinized human epithelium cell. The estimate is done by measuring the (MTT assay) the cell survival rate after the appliation of the product on the surface of the model.
It is possible to evaluate the hard-to-evaluate poorly soluble substance with the monolayer culture system using SIRC.
It is listed on the OECD test guidelines (TG492)

Primary skin irritation tests of three-dimensional models (OECD TG439 method)

We estimate the potential primary skin irritants in a product by using a 3-dimensional cultured epidermis model (J・TEC LabCyte)which is restructured by a normal keratinized human epithelium cell. The product is applied on the surface of the horny layer afterwhich we measure the (MTT assay) cell survival rate.
It is listed on the OECD test guidelines (TG439) as an alternative method for primary skin irritation test using skin as a base material.

Effectiveness tests

Skin lightening

Tyrosinase activity inhibition tests

We evaluate the tyrosinase inhibitory action of the product through the use of a tyrosinase derived from a mushroom.

B16 mouse melanoma cells tests

We evaluate the effect of the product against the melanin production using B16 mouse melanoma.

Skin lightening tests of three-dimension models

We evaluate the effect against the melanin production of a product through the use of a 3-dimensional cultured epithelium model(MatTek corporation MelanoDerm™)This model is restructured by normal human pigment cell and the epithetical cell. The test is done by applying the product on the surface of the horny layer.
It is possible to evaluate the hard-to-evaluate poorly soluble substance with monolayer culture system using B16, or the final formulation.

Anti-aging

Elastase activity inhibition tests

We evaluate the inhibitory action on enzyme activity of the product by using elastases (derived from neutrophil and fibroblast) and artificial substances.

Collagenase activity inhibition tests

We evaluate the inhibitory action on enzyme activity of the product by using collagenases (derived from microorganism and fibroblast (MMP-1)) and artificial substances.

Hyaluronidase activity inhibition tests

We evaluate the inhibitory action on enzyme activity of the product through the use of hyaluronidase derived from bovine testes and artificial substances.

SOD like activity measurement tests

We evaluate the SOD like action (Scavenge action) together with the NBT method by using enzyme reaction and by generating Super oxide.

DPPH radical scavenging activity tests

We evaluate the radical scavenging activity of the product by using 2-2-dipheny-1-picrylhydrazyl (DPPH), which is a stable radical species.

Anti-saccharification activity measurement tests

We measure the activity of the Advanced Glycation End Products (AGEs)-production produced by incubating it with glucose at 37℃ or 60℃ for a predetermined duration. This measures the self-fluorescence of the glycation end product with bovine serum albumin (BSA) as a protein.

Collagen production tests

We evaluate the collagen production activity of the product by ELISA using a normal human fibroblast.

Hyaluronic acid production tests

We evaluate the activity of the product on hyaluronic acid synthesis by ELISA using normal human fibroblast.

Hair growth

Hair papilla cell tests

We evaluate the activity of the product on cell proliferation by utilizing the MTT assay on normal human hair papilla cell.

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